Job Description

This position is responsible for assay development projects relevant to the various vaccine candidates being evaluated at Aeras and collaborates with various groups.  This position requires familiarity with a wide variety of research techniques, statistical analysis, quantitative methods, qualitative methods, writing skills, speaking skills, analytical skills and critical thinking skills.

Essential Duties and Responsibilities

• Culture various strains of recombinant BCG and characterize the strains by SDS-PAGE, Western blotting, PCR, and other quantitative analysis (example; BCA assay or others).
• Optimize and perform Peptide micro-array, Multiplex Cytokine Assay and ELISA for pre-clinical and clinical studies.
• Characterize various recombinant viral vectors by SDS-PAGE, Western blotting, ELISA, qPCR, and other gene/protein expression assays.
• Develop and perform viral titer assays, TCID₅₀, plaque, and focus assays for viral vectors (example; adenovirus, baculovirus, CMV and others).
• Help in producing endogen-free recombinant proteins for assay release development using DNA cloning technology; express recombinant proteins in E. coli and purify using column chromatography.
• Train and qualify colleagues on various protocols, as needed.
• Provide assay support to the Pre-Clinical groups as well as to Process Development and Manufacturing Department.
• Organize and analyze data using computer software when applicable, and interpret results.
• Able to put together the results into technical reports, summaries, and/or graphic presentations.
• Good record keeping for all assay development processes.
• Write, review and edit SOPs.
• Other duties as assigned.

Qualifications

• Bachelor’s or Master’s degree with one to five years related experience and/or training, or equivalent combination of education and experience.
• Experience in cell culture, handling or working with various virus propagation, titer determinations, protein expression and analyses (SDS-PAGE, Western blotting, ELISA), affinity column chromatography techniques, PCR, qPCR, and qRT-PCR.
• Able to communicate effectively, both verbally and written.
• Highly organized, analytical, accurate and detail oriented.
• Able to work under pressure to meet deadlines; able to analyze and prioritize assignments; able to judge the amount of time to spend for the expected outcome according to the importance of a project and the type of information needed.

Rockville, MD, USA

How to apply

Please email your application (cover letter and resume) to

hr@aeras.org

Reference Code

R12-13